Fig 1: SARI inhibits expression of downstream targets in vivo.(a) Staining for VEGF (red) revealed less VEGF-positive cells in SARI than in controlSW480Matrigel plugs, Scale bar, 50 µm; VEGF-positive (%; VEGF positive per total cells; n=5; **P<0.01; Student's t-test). (b) Staining for VEGF (red) revealed less VEGF-positive cells in SARI than in control HCT116 tumours (d), Scale bar, 100 µm; VEGF positive (%;VEGF positive per total cells; n=5; **P<0.01; Student's t-test). (c) Staining for Cp revealed less Cp-positive cells in SARI than in control HCT116 tumours, Scale bar, 100 µm; Cp positive (%;Cp positive per total cells; n=5; **P<0.01; Student's t-test). (d) Staining for HIF-1a (red)-positive cells in SARI and control HCT116 tumours, Scale bar, 100 µm; HIF-1a positive (%; HIF-1a positive per total cells; n=5; **P<0.01; Student's t-test). (e) Immunoblots of VEGF, Cp and HIF-1a expression in SW480-control and SW480-SARI tumours. GAPDH was used as the loading control. (f) ELISA detection of VEGF expression in SW480-control and SW480-SARI tumours (n=3; **P<0.01; Student's t-test). (g) Staining for CD31, VEGF, Cp and HIF-1a in colonic tumours of AOM/DSS induction. Analysis of the number of microvessels, VEGF-positive cells, Cp-positive cells and HIF-1a-positive cells (n=4; **P<0.01; Student's t-test). Scale bar, 100 µm. (h) Immunoblots of VEGF, Cp and HIF-1a expression in colonic tumours of SARI-/- and SARIWT mice after AOM/DSS induction. GAPDH was used as the loading control. (i) ELISA detection of VEGF expression in colonic tumours of SARI-/- and SARIWT mice after AOM/DSS induction. (n=3; **P<0.01; Student's t-test) (j) Nuclear and cytoplasmic proteins were collected from the colonic tumours of SARI-/- and SARIWT mice after AOM/DSS induction. Western blotting was performed to detect Cp expression. Histone H3 was used as the nuclear loading control and ß-actin as the cytoplasmic loading control.